Dihydrotestosterone, Free

Free Dihydrotestosterone (Free DHT) refers to the unbound fraction of DHT that is not bound to proteins (SHBG or albumin) and is directly available to tissues. Free DHT is measured using equilibrium dialysis with LC/MS-MS, which is considered the gold standard method. This measurement is particularly useful when assessing androgen activity, especially in cases where binding protein levels may be abnormal. Free DHT levels correlate with biological activity and can be more clinically relevant than total DHT in certain contexts.